بررسی نقش N – استیل سیستئین در کاهش استرس اکسیداتیو ناشی از دیازینون در کبد و کلیه موش صحرایی

  Background and Objective: Diazinon (DZN) as an organophosphate (OP) pesticide induces the production of free radicals and oxidative stress. The aim of this study was to investigate the effect of N-acetyl cysteine (NAC) as a n antioxidant against DZN- induced oxidative stress in rat liver and kidney.   Materials and Methods: In the present experimental study , male Wistar rats were randomly divided into four groups including: control (vehicle, corn oil), DZN group (100 mg/kg), NAC group (160 mg/kg), and experimental group: co-treatment with NAC and DZN. Twenty four hours after injection, animals were anesthetized by ether, and then liver and kidney tissues were quickly removed. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, and the levels of glutathione (GSH) and malondialdehyde (MDA) were measured by biochemical methods. The data was statistically analysed using analysis of variance (ANOVA) followed by post hoc analysis using Tukey tests.   Results: DZN increased SOD, CAT and GST activities in liver and kidney and LDH activity in liver. The decreased GSH content and increased MDA level in liver and kidney were also observed. Administration of NAC significantly decreased SOD, GST and LDH activities in liver and decreased CAT activity in liver and kidney. NAC increased GSH level in liver and kidney and decreased MDA level in liver.   Conclusion: Diazinon probably causes free radical production, which leads to the enhanced lipid peroxidation membrane and depleted GSH content in liver and kidney. Administration of NAC by directly eliminating ROS and GSH synthesis and decreasing DZN toxicity, but it does not protect completely.   Key words: Diazinon, NAC, Oxidative stress, Liver, Kidney, Rat     Funding: This work was supported by a grant from Chemical Injuries Research Center of Baqiyatallah University of Medical Sciences (Tehran, Iran).   Conflict of interest: None declared.   Ethical approval: The Ethics Committee of the institute approved the experimental protocol and all efforts were made to minimize the animal suffering    How to cite this article : Izadi F, Jafari M, Bahadoran H, Asgari A.R, Divsalar A, Salehi M. The role of N-acetyl cysteine on reduction of diazinon-induced oxidative stress in rat liver and kidney . J Rafsanjan Univ Med Sci 2014 12(11): 895-906. [Farsi]